Journal: Pathogens

Article Title: Impact of Neospora caninum Infection on the Bioenergetics and Transcriptome of Cerebrovascular Endothelial Cells

doi: 10.3390/pathogens9090710

Figure Lengend Snippet: Top differentially expressed genes (DEGs) detected in human and rat endothelial cells after 24 h of N. caninum infection.

Article Snippet: Rat Brain Microvascular Endothelial Cell line (rBMVEC), GPNT strain, was obtained from the European Collection of Authenticated Cell Cultures (ECACC, Health Protection Agency Culture Collections (HPACC), Salisbury, UK).

Techniques: Infection, Ubiquitin Proteomics, Membrane, Binding Assay, Transduction

Journal: Pathogens

Article Title: Impact of Neospora caninum Infection on the Bioenergetics and Transcriptome of Cerebrovascular Endothelial Cells

doi: 10.3390/pathogens9090710

Figure Lengend Snippet: The effects of Neospora caninum on the expression levels of the genes of hBMECs and rBMVECs determined by microarray analysis at 24 hpi. PCA scatter plots of gene expression profiles of ( a ) hBMECs and ( d ) rBMVECs showing a clear infection-dependent effect in both cell lines. The plots demonstrate a clear separation in the expression profile of infected and uninfected (control) cells. ( b , e ) Volcano plots showing individual genes as dots. The red and blue dots represent genes with increased and decreased expression, respectively. Genes whose expression levels were not significantly changed are shown in grey. X -axis shows Fold Change (FC) and y -axis shows p -value. ( n = 3/group; p -value < 0.05; FC ± 1). ( c , f ) Heat map showing hierarchical clustering of DEGs between infected and uninfected hBMECs and rBMVECs at 24 hpi. Each group had three replicates. Red and blue colors indicate genes with increased and decreased expression, respectively. Gene expression in infected cells was compared to uninfected cells and the criterion for differential expression was ≥ 1FC with a p -value of < 0.05.

Article Snippet: Rat Brain Microvascular Endothelial Cell line (rBMVEC), GPNT strain, was obtained from the European Collection of Authenticated Cell Cultures (ECACC, Health Protection Agency Culture Collections (HPACC), Salisbury, UK).

Techniques: Expressing, Microarray, Gene Expression, Infection, Control, Quantitative Proteomics